![]() ![]() They can be produced, in a autotrophic manner and on non-agricultural land, be cultivated in seawater or wastewater and offer a higher lipid productivity than first-generation biofuels. Microalgae are currently considered as potential actors for the third biofuel generation for several reasons. Moreover, the overall conclusion of the present review study gives limitations on the use of fluorochrome for screening of lipid-rich microalgae species and suggests improved protocols for staining recalcitrant microalgae and recommendations for the staining quantification. The available literature addresses the limitations of fluorescent dyes under certain conditions, such as spectral properties, dye concentrations, cell concentrations, temperature and incubation duration. This review collates and presents recent advances in algal lipid staining and focuses on Nile red and BODIPY 505/515 staining characteristics. Their fluorescent staining for lipids offers a rapid and inexpensive analysis tool to measure neutral lipid content, avoiding time-consuming and costly gravimetric analysis. For this purpose, the fluorescent dyes most commonly used to quantify lipids are Nile red and BODIPY 505/515. Major challenges for the development of this resource are to select lipid-rich strains using high-throughput staining for neutral lipid content in microalgae species. ![]() In addition, they do not compete for arable land and offer very high lipid yield potential. ![]() Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. ![]()
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